天狼猩红―偏振光法检测子宫内膜异位症中Ⅰ，Ⅲ型胶原.doc

【关键词】子宫内膜异位症关键词:子宫内膜异位症;胶原;荧光偏振;天狼猩红摘要:目的探讨子宫内膜异位症中Ⅰ,Ⅲ(EM)患者的异位内膜组织(红色病变组和黑色病变组)和11例非子宫内膜异位症患者的在位内膜组织(对照组)用天狼猩红染色,偏振光观察,photoshop分析,半定量研究Ⅰ,ⅢⅠ,Ⅲ型胶原在对照组中含量最低(P<,P<),Ⅲ胶原在红色病变组中含量最高(P<),Ⅰ型胶原在黑色病变组中含量最高(P<).Ⅰ,Ⅲ型胶原在增生期和分泌期无明显差异(P>).结论子宫内膜异位症患者的异位组织中有Ⅰ,Ⅲ型胶原的表达.Ⅰ,Ⅲ┐polarizationdeterminationoftypeⅠ,ⅢcollageninendometriosisimplantLIJie,XINXiao-Yan,CUIGeng1DepartmentofGynecology&Obstetrics,2DepartmentofOrthopedics,XijingHospital,FourthMilitaryMedicalUniversity,Xi'an710033,ChinaKeywords:endometriosis;collagen;fluorescencepolariza-tion;siriusredAbstract:ypeⅠ,Ⅲcollagenpresentinectopicendometriumandintheconnectivetissuesurround-ingectopicendometrialimplant,,redlesionandblacklesionwerecollectedandtypeⅠ,Ⅲ-centageofthetotalstainedareaamongthethreegroups(A,B,C).AmountofⅠ,ⅢtypecollagenwasthelowestingroupC(P<,P<),andthestainedareaoftype-ⅢcollageningroupAwasthehighestamongthethreegroups(P<).Theamountoftype-Ⅰcollagenwassig-nificantlyhigheringroupBthaningroupAandgroupC(P<).CONCLUSIONThisstudydemonstratesthepresenceoftypeⅠ,Ⅲcollagenintheintrauterineandec-Ⅰ,Ⅲurrenceanddevel-,约有10%的育龄妇女患有子宫内膜异位症(Endometriosis,EM)[1],[2]提出的经血逆流种植理论,根据这一理论,在子宫内膜种植和增生之前,它们必须到达盆腔,,子宫内膜细胞必须与腹膜建立细胞-细胞或细胞-,EM早期损伤中最初脱落的细胞可侵入腹膜的胞外基质(extracellularmatrix,ECM)[3].ECM是一种活泼的动态物质,它们影响着细胞的分化、增殖、粘附、形态发生和表型表达的生物学过程,因而与组织修复、肿瘤侵润转移、[4],―偏振光方法(Pi-crosirius-Polarization)半定量检测Ⅰ,Ⅲ型胶原在EM患者和正常育龄妇女中的表达情况,-01/2000-,A组为红色病变期12(增生期7,分泌期5)例(病