聚丙烯酰***凝胶的蛋白质,重复性好,能在蒸馏水中保存数月条带也不会消失,成本较低,操作简便,无毒灵敏度最高()灵敏度较高(1-10ng),简单快速(30-60min)蛋白质保存完整,简单快速灵敏度相对较低,结果容易受到核酸的污染,背景色较高背景较高,有毒,费时费力(8-16h),成本高,操作复杂使蛋白质变性,需要紫外***,multistepprocess,-purityreagentsandprecisetimingarenecessaryforreproducible,high-:Treatwithacid,,suchasbuffers,ions,denaturants,detergents,orcarrierampholytes,:Treatwithreagents,thisrendersproteinsmorereactivetowardsilver,:.(i)plexofsilvernitrateinahighlyalkalineenvironment(ammoniaandsodiumhydroxide).Development(显影):diluteacidicsolutionsofformaldehyde.(ii)Theacidicmethodsusesilvernitrate(weaklyacidicsolution).Development::Ag++le-→:(plexationdecreasestheredoxpotentialandthusthereducibilityofsilver.(ii):-dependent:RED+mOH-→OX+nH2O+me-+2OH-→HCOOH+H2O+2e-:(i)free;(plexedtothepolyacrylamidegel;(plexedtoproteins,
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