姜黄素对大鼠肺微血管平滑肌细胞增殖的影响.doc

姜黄素对大鼠肺微血管平滑肌细胞增殖的影响
复旦大学附属中山医院麻醉科邮编200032
张鹏黄海琼姜桢
【摘要】目的:研究姜黄素对大鼠肺微血管平滑肌细胞增殖的影响。方法:实验分对照组和不同浓度姜黄素处理组。药物作用24小时后,MTT法测姜黄素对大鼠肺微血管平滑肌细胞增殖的抑制作用,流式细胞仪检测姜黄素作用后细胞周期变化。结果:姜黄素以浓度依赖的方式抑制肺微血管平滑肌细胞增殖;流式细胞仪分析显示,姜黄素处理过的细胞S期明显减少,主要停滞在G0/G1期。结论:姜黄素可以抑制大鼠肺微血管平滑肌细胞增殖,呈现浓度依赖性,其作用可能是通过阻滞细胞周期进程实现的。
关键词:姜黄素;肺小血管平滑肌细胞;细胞周期
Effect of curcumin on proliferation of rat pulmonary microvascular smooth muscle cells
【Abstract】Aim: To explore the effect of curcumin on proliferation of rat pulmonary microvascular smooth muscle cells. Methods: The inhibitory rate of rat pulmonary microvascular smooth cells in different concentrations of curcumin were measured by MTT assay. Cell cycle was detected by flow cytometry. Result: The inhibitory effect of curcumin on rat pulmonary microvascular smooth cells was showed in a dose-dependent manner(P﹤). Cell cycle analysis showed reduced percentage of cells in S phase and G0/G1 phase arrest(P﹤).
Conclusion: Curcumin exerts inhibitory effect on proliferation of rat pulmonary microvascular smooth muscle cells in a dose-dependent manner possibly through induing cell cycle arrest.
Key words: curcumin; pulmonary microvascular smooth muscle cells; cell cycle
肺动脉高压发病中病理学改变主要在肺小动脉1,肺小血管出现明显的重构,内膜、中膜及外膜的内皮细胞,平滑肌细胞和成纤维细胞异常增殖,各层细胞间基质沉积2。如何抑制肺血管细胞的异常增殖,降低肺动脉压力成为治疗肺动脉高压的一项重要措施。姜黄素,中药姜黄中提取的一种酚性色素,常作为调味品及染料。近年来的研究显示在心血管系统中具有明显的保护作用,抑制主动脉平滑肌细胞的增殖3。本实验研究姜黄素对大鼠肺微血管平滑肌细胞增殖的