Prolactin and the Secretion of Insulin and Glucagon by the Pancreas 1955 Piero P. Foà.pdf

该【Prolactin and the Secretion of Insulin and Glucagon by the Pancreas 1955 Piero P. Foà 】是由【阳仔仔】上传分享，文档一共【4】页，该文档可以免费在线阅读，需要了解更多关于【Prolactin and the Secretion of Insulin and Glucagon by the Pancreas 1955 Piero P. Foà 】的内容，可以使用淘豆网的站内搜索功能，选择自己适合的文档，以下文字是截取该文章内的部分文字，如需要获得完整电子版，请下载此文档到您的设备，方便您编辑和打印。ProlactinandtheSecretionofInsulinandGlucagon
bythePancreas1
,GIORGIOGALANSIN02ANDERMINIOCOSTA3
FromdheDepartmentofPhysiologyandPharmacology,TheChicagoMedicalSchool,Chicago12,Illinois
NDERcertainexperimentalconditions,animalprolactinmaystimulatethesecretion
prolactinhasadiabetogenicactionofanti-insulinhormonesofpituitary,adrenal
U(1-4)whichseemstobeaccompa-
niedbyadecreasedinsulincontentofthepan-thispaperwasdesignedtostudytheeffects
creas(5).Experimentsperformedinthislabo-ofprolactinonthesecretion(orliberation)of
ratory(6)havedemonstratedthatthefirstinsulinandofglucagonbythepancreas.
injectionofprolactininnormaldogscauses
aloweringofthebloodsugarassociatedwithMETHODS
amarkeddegranulationoftheBcellsoftheTheproblemwasinvestigatedbymeansof18cross-
circulationexperiments,using36healthymongreldogs
isletsofLangerhansandthatrepeatedin-
jectionsarefollowedbyhyperglycemiawithhoursbeforetheexperiment,wereanesthetizedwith
(35mg/kgintraperitoneally),
depancreatizeddogs,prolactinalwayscausesheparinizedsandpreparedasdescribedinprevious
,al-papers(9-1I).In15experiments,thepancreatic-duo-
denalveinofadonordogDwasconnectedwitha
thoughprolactinitselfmayhaveahypergly-
cemiceffect,itsinjectionintoanormaldog,wereofthreetypes:infiveexperiments,untreated
forthefirsttime,causestheliberationofpre-donordogsreceivedanintravenousinjectionofpro-
formedinsulininamountssufficienttolowerlaCtin5(ArmourLot%4911~4;20m/kg),atotime;
infiveexperiments,thedonordogsreceivedthreepro-

injections,theactivityoftheBcellsisreducedandafourthoneduringtheexperiment,atotimein
andprolactinhyperglycemianolongercanbefiveexperiments,untreateddonordogsreceivedIuof
,in-PituitrinS,anamountsimilartothatpresentasim-
-
sulincouldnotbesecretedatallandhyper-ingthreeexperiments,theanastomosiswasmadebe-
glycemiawouldresult,
,untreateddonors
time,althoughitsoccurrenceinhypophysec--
minedinduplicatewiththemethodofNelson(I2).The
tomized,adrenalectomizedanddepancreatizedstatisticalsignificanceoftheresultswascalculated
animals(3-6)suggeststhat,likeanteriorpitu-accordingtoFisher(13).
itarygrowth(diabetogenic)hormone(7,s>,
prolactinmayactdirectlyontheuptakeofRESULTS

theadditionalpossibilitythatinthenormalaveragechangesintheconcentrationofblood
glucosecomparedtothepre-injectionvalues.
ReceivedforpublicationMarch17,1955.
FigureIrepresentsthechangesinbloodsugar
lThesestudieswereaidedbygrantsfromtheUnited
-observedinfivepancreatic-femoralcross-circu-
-
-treateddonordogsreceivedaninjectionof
dress:IstitutodiAnatomiaPatologica,
Milano,Italy.
3ResearchFellow(Fulbright).Permanentaddress:precedingtheinjection,therewerenosignifi-
IstitutodiFarmacologia,UniversithdiCagliari,Italy.
4Theproductionofpermanentdiabetesbypro-,
longedtreatmentwithprolactinhasnotbeenat--
,
${}${}()onDecember23,2018.
,
P-PROLACTINP-PROLACTIN
D-ANIMALSD-ANIMALS
DiSCONNECTEDDISCONNECTED
O-DONOR
O-DONOR
O-RECIPIENT
II1
QL-40’
5-600+60+120+1803-600t60+120+180
MlNUfESMINUTES
FIG,
-femoralanastomosis,Fiveexperi---
.
cantchangesinthebloodsugarofeitherdugDDISCUSSION

intodogD,thebloodsugarofbothdogsbeganobservedindogsDandRfollowingthefirst
todeclinesignificantly(P<)reachingainjectionofprolactinintodogDisduetothe
minimumafterabout45minutesintherecip-secretionortotheliberationofinsulinbythe
ient,andcontinuingtodeclineafterinterrup-pancreasofdogDandtoitspartialtransfer
-femoralanas-
zrepresentstheresultsofthreecontrolmesen-
teric-femoralcross-(6),this
theseexperiments,theinjectionofprolactinhypoglycemiaprobablyisduetoliberationof
intopreviouslyuntreateddonordogsagainpreformedinsulinratherthantoitsincreased
causedamarkedandsignificant(P<)
hypoglycemia,butinthiscase,thebloodsugaritimprobablethatthefallinbloodsugarof
oftherecipientdogsremainedessentiallydugRisduetotransferofsignificantamounts
:a)the
Rreceivingmesenteric
obtainedinfivepancreatic-femoralcross-circu-bloodsugarofdug
insteadofpancreaticbloodremainsunchanged,
lationexperimentsinwhichdonordogsprevi-
andb)thebloodsugarofdogRreturnstoward
ouslytreatedwithprolactinreceivedanaddi-
normalshortlyaftertheflowofinsulin-carry-

ingpancreaticbloodfromdogDisinterrupted,
experiments,prolactincausedamarkedandwhilethebloodsugarofdogD,whichisunder
significant(P<)hyperglycemiaindogDtheinfluenceofprolactin,continuestofall.
accompaniedbyamoderateandpossiblysig-Afterrepeatedinjections,prolactincausesa
nificant(P=)
-
fivepancreatic-femoralcross-circulationexper-geststhatthehyperglycemiaofdogDisnot
imentsinwhichuntreateddonorsreceivedantheresultofanincreasedsecretionorlibera-
,onthecontrary,
inthebloodsugarofdogDandonlyamoder-itisaccompaniedbythesecretionofsmall
ateandpossiblysignificant(P=)hypo-amountsofinsulincoincidentwiththepartial
(6).Partof
${}${}()onDecember23,2018.
PROLACTINANDINSULINSECRETION495
w+40
P-PlTUITRlN-S
08
DISCONNECT‘ED3D-ANIMALS
WDlSCONNECTED
+20
3-600t60t120t180
MINUTESMINUTES
-
Donordogspretreatedwithprolactin,recipientdogsun--
--experiments.
ments.
thisinsulinistransferredtodogRwhereitdenalormesentericveinofthedonordogD
causeshypoglycemia,
counteractthehyperglycemiceffectofprolac-intravenousinjectionofprolactinintoprevi-
-
inthepancreaticbloodofdogstreatedwithingofthebloodsugarassociatedwiththe
prolactin,controlmesenteric-femoralcross-cir-appearanceofahypoglycemicmaterial(in-
culationexperimentswithpretreateddonorssulin),butnot

posteriorpituitaryhormonesalsomaycausethroughtheanastomosisandcauseshYPoglY-
hyperglycemia(7,14).
elevationinbloodsugarcanbeobtainedonlyindonordogspreviouslytreatedwithrepeated

thanthosecontainedintheprolactinprepara-material(glucagon)appearsinthepancreatic
tionused,thepossibleinfluenceoftheseim-
puritiesontheresultswasinvestigatedandthehypothesisthattheso-calleddiabetogenic
ruledoutbytheexperimentswithPituitrineffectofprolactinmaybetheresultofarapid
andbycontrolexperimentswithACTHpre-dumpingofpreformedinsulinbytheBcells
viouslyreported(6).Similarly,anyeffectsofoftheisletsofLangerhansfollowedbyde-

outbytheresultsofthemesenteric-femoralsuggeststhatprolactindoesnotcausean
cross-circulationexperimentsandofexperi-increaseinglucagonsecretion.
mentswithsalinepreviouslyreported(9).TheREFERENCES
slightfallinthebloodsugarofdogRfollowing
,
theinjectionofPituitrinintodogDoccurs
45:627,=949*
alsoaftertheinjectionofsaline(9),.
:roe,1953.
,
:23,1953.
SUMMARYANDCONCLUSIONS4,HOUSSAY,.,-
:33,1953.
Eighteencross-,C.,,.
performedanastomosingthepancreatic-duo-:260,1951.
${}${}()onDecember23,2018.
&
,.,,,.,,,GLASSIAANAND
:313,,;..&A&:
,:758,=953*
471,I953-,:375,1944.
,:649,
,&s&xlMethodsj’wResearchWork-
*9s*
,.,:Oliver&Boyd,Ltd.,1938.
:197,,.,,,-
,.,,H,,,,~.
:32,UzcmavtPj?ys%‘uZogy(2nded,).NewYork:McGraw-
1952*Hill,1955,p*4239
${}${}()onDecember23,2018.