2010全国生物材料大会论文集之 (274).pdf

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Biotransformation of DL-2-Amino-∆-Thiazoline-
-4-Carbonic Acid to L-Cysteine Using a Newly
Isolated Pseudomonas sp. Zjwp-14
Pu Wang* Guoying Lv Junyao He Weiqing Chen
College of Pharmaceutical Science College of Biological and Environmental Engineering
Zhejiang University of Technology, 310032, Zhejiang Shuren University, 310015,
Hangzhou, . China Hangzhou, . China
******@zjut.


Abstract- A bacterial strain Pseudomonas sp. Zjwp-14 was hydrolase (ATCase), and (3) hydrolysis of S-carbamoyl-
screened out from soil, and used for the biotransformation of cysteine (or N-carbamoylcysteine) with amidohydrolase.
DL-2-amino-∆2-thiazoline-4-carbonic acid to L-cysteine. This In the present study, we reported the isolation and
strain was identified based on its morphological, physiological characterization of a newly isolated bacterial strain, which is
characterization, 16S rDNA sequence determination and capable of producing L-cysteine with 10 g·l-1 of DL-ATC as
ic analysis. The bioconversion process was optimized substrate. The substrate concentration used for this strain is
by examining some key factors including cell cultivation time, higher than previous reports[3,5]. The strain was identified as
buffer pH, metal ions, aeration (anaerobic/aerobic) and substrate Pseudomonas sp. Zjwp-14 based on its morphological,
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concentration.