SD大鼠肝癌模型建立及病理分析论文.doc

SD大鼠肝癌模型建立及病理分析
学院名称： 生命科学学院
专业名称： 生物科学
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SD大鼠肝癌模型建立及病理分析
摘要：本实验采用灌胃的方法构建诱发性肿瘤模型，将二乙基亚硝***（Diethyl nitrosamine，DENA）配成1%水溶液，根据每只SD雄性大鼠称重结果确定灌药量，70mg/kg，每周1次，灌胃14次之后正常饲养。从灌胃之日起，分别在第2、4、8、11、15、16周分六组处死大鼠，完整取其肝脏称其重量，计算肝体比；HE染色制作病理切片观察癌变演进中肝组织结构变化。结果显示，大鼠肝体比实验组与对照组相比，从第8周开始二者呈显著性差异（P<），第11-16周二者呈极显著性差异（P<）。病理切片结果表明成功获得大鼠肝轻度变性坏死、重度变性坏死、结节性增生、肝硬变、肝癌（包括肝细胞癌及肝胆管癌）五个时期的组织材料，病理演变过程和人类肝癌发生过程相似。克服了目前国内外对肝癌研究多取材于临床中晚期标本的局限性，为更加全面系统地研究肝癌演进的发生发展及分子机理奠定了坚实的基础。
关键词：大鼠；肝癌动物模型；诱发性肿瘤模型；二乙基亚***
SD Rat Hepatocarcinogenesis Model and Pathological Analysis
ZHU Bao-min
Abstract In this study, we use the gavage method to construct induced tumor model, diethylnitrosamine (DENA) paired 1% solution, according to SD male rats each weight to determine irrigation dose, 70mg/kg, 1 week, after 14 times to conduct normal feeding . The rats were devided into six groups and killed on the 2th week, 4th week, 8th week 11th week, 15th week, 16th week respectively when the rats were piped drinking, and the liver of every rat was taken completely then weight them and calculate the hepatosomatic. Structural changes in liver tissue was observated by HE staining. The results showed that the hepatosomatic of the experimental group compared to the control group, from the 8th week showed a significant difference (P<), 11-16th weeks showed significant differences (P<). According to the pathologic slices, liver degeneration and necrosis, severe degeneration and necrosis, nodular hyperplasia, cirrhosis, liver cancer (including hepatocellular carcinoma and liver bile duct) were got successfully, the evolution of pathology was similar process with human liver cancer.
Overcome the limitations of the current domestic and international research on liver cancer taken from late clinical specimens, for a more comprehensive and systematic study of the incidence of liver cance