GSTM1基因多态性与AFB1-DNA加合物水平的关系.doc

GSTM1基因多态性与AFB1-DNA加合物水平的关系GSTM1基因多态性与AFB1-DNA加合物水平的关系作者:黄永秩,班副植,周远峰,梁秋翔,易芸,范露丹,姚利民,龙喜带【摘要】目的探讨解毒酶基因谷胱甘肽S-转移酶M1(GlutathioneS-transferaseM1,GSTM1)多态性对黄曲霉***B1(aflatoxinB1,AFB1)高污染区广西人群AFB1-DNA加合物水平的影响。方法采用竞争性酶联免疫吸附法检测研究对象(966例)AFB1-DNA加合物水平,而GSTM1基因型分析则通过PCR-RFLP来完成。结果研究结果显示GSTM1缺失型(GSTM1-null)与高AFB1-DNA加合物水平相关,其校正风险值(OR)(95%可信区间,即95%,)。结论AFB1在带有易感基因型GSTM1-null的人群更易诱导产生DNA损伤(AFB1-DNA加合物)。【关键词】AFB1;AFB1-DNA加合物;GSTM1;多态性Abstract:ObjectiveToinvestigatetheeffectsofGlutathioneS-transferaseM1(GSTM1)polymorphismonAFB1-DNAadductsamonginhabitantshighlyexposuretoaflatoxinB1(AFB1)-linkedimmunosorbentassaywasusedtomeasuretheAFB1-DNAadductslevelsin966participants,pletedbyusingPCR-(GSTM1-null)ispositivelyassociatedwithelevatedAFB1-DNAadducts(ajustedoddsratio,OR=,95%confidenceinterval,95%CI=,).ConclusionAFB1easilyresultedinDNAinjuries(AFB1-DNAadducts)amongpopulationsusceptibletoGSTM1-:AFB1;AFB1-DNAadducts;GSTM1;polymorphism黄曲霉***B1(aflatoxinB1,AFB1)是由黄曲霉菌产生的一种人类致癌物,其在体内经细胞色素P450(cytochromeP450,CYP)的作用转变为基因毒性产物8,9-环氧AFB1(8,9-epoxideAFB1,AFB1-epoxide),后者可与DNA结合形成AFB1-DNA加合物,该加合物可经解毒酶或DNA修复酶清除或修复[1,2]。谷胱甘肽S-转移酶M1(GlutathioneS-transferaseM1,GSTM1)是一种重要的解毒酶,通过催化谷胱甘肽结合外源性毒物而在二相生物转化过程中占重要地位[3]。以往研究表明该基因的缺失型(即GSTM1-null)与AFB1相关性肝细胞癌(hepatocellularcarcinoma,HCC)发病相关,提示GSTM1功能的缺失